Pharmacy & Drugs
Manuel de Expérimental Pharmacology
Description
This book provides fundamental knowledge de practical aspects de le experimental pharmacology right from laboratory animals handling et tissue mounting to practical implications de various important complex experimental procedures. This book is prepared using simple language et tried to explain le experiment avec a model data de observation, calculation, graph et result pour better clarity to carry out le experiments en animals or understand le experimental pharmacological techniques by observing le simulated experiments. Exact simulation de real animal experiments sur a computer is not easy because le biological responses are very complex. Moreover, imagination de experimental outcome et acquiring le skill de handson experiment is not satisfactorily learned by observing le effect de le drug en simulated experiments. This book helps to reduce le gap between hands-on experiments en animal et simulated experiments en pharmacology. Le experimental methodology et experimental data explained en this book are designed sur le basis de robust scientific materials et personal experience en hands-on experiments under le guidance de eminent personalities. This book will be helpful pour graduates et postgraduates related to pharmacology, trainees, research workers during their day-to-day activities i ncluding allied health discipline et scientists en industrial drug discovery set-up et CRO. Several simple et newer experimental models have been incorporated which will help le students to engage en drug discovery activities en future. Besides this, several important points have been discussed e.g. ethics de animal experimentation, care et handling de experimental animals, preparation de solutions, tissue mounting pour en vitro studies etc.
Table des matières
À propos de le Author -Acknowledgements -Preface -Basics de Expérimental Pharmacology -1. Introduction à experimental pharmacology –2. Commonly used instruments en experimental pharmacology –3. Commonly used laboratory animals en experimental –pharmacology4. CPCSEUn guidelines pour laboratory animal facility –5. Handling et restraint de small laboratory animals –6. Routes de drug administration en laboratory animals –7. Techniques de blood collection en laboratory animals – 8. Anaesthetics used en experimExperiments sur Isolated Preparations (Ex-Vivo) –10. Physiological salt solutions (PSS)used en isolated tissue –preparation11. Procedure pour recording isolated tissue responses –12. Dose-response curve (DRC) de acetylcholine using frog –rectus abdominis muscle.13. Effect de physostigmine sur DRC de acetylcholine using frog –rectus abdominis muscle14. Effect de atropine sur DRC de acetylcholine using rat ileum –15. Effect de spasmogens et spasmolytics sur rabbit jejunum/ –Effect de drugs sur gastrointestinal motility16. Effect de agonist et antagonists sur guinea pig ileum –17. Effect de saline purgative sur frog intestine –18. Determination de PA2 value de prazosin using rat –anococcygeus muscle19. Determination de PD2 value using guinea pig ileum –preparation20. Effect de drugs sur isolated frog heart preparation –ental pharmacology –9. Techniques de euthanasia used en experimental –pharmacology-Bioassays – 21. Introduction à bioassay –22. Bioassay de histamine using guinea pig ileum by matching –method 23. Bioassay de oxytocin using rat uterine horn by interpolation method 24. Bioassay de serotonin using rat fundus strip by three-point – method25. Bioassay de acetylcholine using rat ileum by four-point –method 26. Bioassay de atropine using guinea pig ileum by –interpolation method 27. Bioassay de d-tubocurarine by rabbit head-drop method – 28. Bioassay de digitalis by guinea pig cardiac arrest method – 29. Bioassay de insulin by rabbit hypoglycemic effect method – 30. Bioassay de heparin sodium by sheep blood coagulation method In-Vivo Experiments en Animals –31. Effect de drugs (miotics, mydriatics et local anaesthetics) sur rabbit eye 32. Effect de drugs sur ciliary motility de frog oesophagus –33. Skeletal muscle relaxant property de drug using rota-rod – apparatus 34. Skeletal muscle relaxant property de drug using hanging wire test 35. Local anaesthetic activity de drugs by different methods 36. Effect de drugs sur locomotor activity en mice using actophotometer 37. Analgesic activity de drugs using central et peripheral methods 38. Anti-inflammatory activity de drugs using carrageenan induced paw-oedema model 39. Anti-ulcer activity de a drug using pylorus ligated (SHAY) rat model 40. Anti-ulcer activity de a drug using NSAIDS induced ulcer model 41. Effect de sulfasalazin sur indomethacin-induced inflammatory bowel disease en rats 42. Anti-allergic activity by mast cell stabilization assay – 43. Effect de finasteride sur testosterone-induced prostatic hyperplasia en rats 44. Hypoglycemic effect de insulin en rabbits –