Horticulture et Planteation Cultures
Techniques en Diagnosis de Plante Viruses Vol. 6
Description
Diagnosis de plant viruses can be difficult when one is dealing avec unstable viruses, unusual strains, or viruses infecting woody plants. Méthodes used en virus diagnosis include observation de symptoms sur inoculated indicator plants, detection de virus-induced cellular inclusions by light or/and electron microscopy, test de le vector capability de certain insects, serology, et detection de le viral nucleic acids. Le usefulness de each method depends sur le virus et le host. Diagnosis based sur le use de indicator plants is not reliable because plant reactions et symptoms vary depending sur environmental conditions, plant cultivars, et virus strains. Other methods, such as electron microscopy, tests de vector capability, et detection de viral nucleic acids by means de radioactive probes, may not be practical pour some laboratories. Serology is le most widely used method pour virus diagnosis. Some new techniques include immunological methods, electrophoretic analysis de dsRNUn profiles, molecular hybridization, PCR, and/or reverse transcription (RT)-PCR. In most de these assays, usually one pathogen is detected per assay. DNUn microarrays have le ability to simultaneously display le expression de thousands de genes at a time, thus it is a powerful tool pour genetic analysis. Other techniques such as RT-PCR using degenerate primers et dsRNUn as template, le use de dsRNUn analysis as detection tool, detection de viruses belonging to different families avec a single pair de PCR primers, le use de monoclonal antibodies, et le use de spot nested RT-PCR et degenerate dl-containing primers are discussed. This book is part de le multi-volume Plante Pathogens Series. Le present volume contains 13 chapters sur different techniques being frequently used by scientists worldwide en diagnosing plant viruses
Table des matières
Techniques en Diagnosis de Plante Viruses, dsRNUn as a Detection et Diagnostic Tool pour Plante Viruses, Sensitive et Specific Detection de Cucumber mosaic vims, Detection et Strain Differentiation de Soybean dwarf vints by Nucleic Acid Techniques, RT-PCR using Degenerate Primers et dsRNUn Template pour Detection de Plante Viruses, Generic Detection et Differentiation de Viruses using a Spot Nested RT-PCR et Degenerate dI-containing Primers, Detection de Cymbidium mosaic vims et Odontogloss ringspot vints Using a Single Pair de PCR Primers, Moléculaire Diagnostics pour Detection de Virus et Virus-like Pathogens en Citrus, Diagnosis de Tomato Mosaic Tobamovirus Using Monoclonal Anti bodies, Diagnosis de Begomoviruses, Characterization et Diagnosis de Viruses Occurring sur Olea europaea L., DNUn Micro arrays: Technologie, Applications et Potential Applications pour le Detection de Plante Viruses et Virus-like Pathogens, Moléculaire Diagnostics de Virus-vector Nematodes, Application de Resistance Gene Analogs en Breeding pour virus resistance.