Plant Protection
Diagnosis of Plant Viruses and Allied Pathogens
Description
For the past 35 years I have been working on viruses diseases of plants, the characterization of associated viruses, their detection by biological, serological and molecular techniques. After 2005, it was my privilege to offer short and long courses (group and hands-on training) on plant viruses, their characterization and development of diagnostics. These courses were provided to university teachers, research organizations, Government officials, senior professors and Quarantine scientists. During these trainings, most of the trainee scientists desired that a compilation with detail protocols should be developed. With teaching, research and trainees requirement in mind, this book has been written to provide detail protocols of each and every experiment related to plant virology based on the experience, I had gained working on plant virology.
Table of Contents
introduction; Symptoms Caused by Viruses and Virus-Like Pathogens; Transmission; Host Range and Properties of the Virus in Crude Extract in vitro; Virus Isolation and Purification; Electron Microscopy; Serology; Immunosorbent Electron Micro-scopy (ISEM); Nucleic Acid Based Diagnosis; Viroids and their Molecular Diagnosis; Cloning and Sequencing; Microarrays - An Introduction; Virus Eradication from Plants; Taxonomy of Viruses - An Introduction; Selected Reading; Selected References; Internet Search; Appendix I; Appendix II; Appendix III; Appendix IV; Standard pH Solutions; Approximate pKa Values of Substances, Useful for Buffer Systems; Conversion Tables; Number of Trees per Acre at Given Spacings; Number of Trees Per Hectare at Given Spacing; Index