Biotechnologie et Génétique Ingénierie
Monoclonal Antibodies Application en Disease et Health
Description
Inde is likely to become a major force en le area de Biotechnologie en le next decade. Le Department de Biotechnologie has encouraged human resource development by creating new institutions et centres de excellence pour le research et training. Any research work en biotechnology uses Hybridoma Technologie et Antibody Ingénierie as a basic tool en manipulation de cell organelle genetically as well as phenotypically. This book is an attempt to comprehensively present le information sur Hybridoma Technologie et Antibody Ingénierie en single volume. It is intended primarily as a text book pour undergraduate et post graduate students majoring en Biotechnologie, Biosciences, Biotechnologie product formulations, preparation de vaccines et diagnostics used en veterinary et human medicine. Le new biology is being inducted through animal cell culture et hybridoma management into animal industry. This book has eleven chapters that deal avec historic developments, laboratory design, sterilization procedures et various facets de hybridoma culture. This includes preservation, characterizations, storage et transport de cells, their monitoring et technologies pour species cell culture.
Table des matières
À propos de le Series; Preface; Introduction: Histoire de hybridoma technique; Principes de monoclonal antibody production de predefined specificity; Monoclonal antibodies–an overview; On proteins vs nucleic acids; Structure de proteins; DNUn et protein electrophoresis; Antigens et antibodies–structure et function-a recap; Antigen–antibody interactions; Requirements de a Hybridoma Laboratory: Introduction; What is cell culture?; Mice facility; Production de Monoclonal Antibodies: Introduction; Collection de spleen; Myeloma cell preparation ; Fusing agents; Components de HAT medium; Fusion de spleen lymphocytes et myeloma cells; Cloning by limiting dilution; Hybridoma cells; Taggert hybridoma technology; Naming le hybridoma clones; Immunoassays pour screening de mAbs; Neutralization assays; Radio immuno assay; Characterization de Monoclonal Antibodies: Polyacrylamide gel electrophoresis; Isoelectric focusing; Autoradiography et fluorography; Western blotting; Specificity de le monoclonal antibodies; Isotyping de monoclonal antibodies; Affinity de le mAbs; Characterization de le mAbs pour intended use; Selection de mAbs; Mass Production de Monoclonal Antibodies: Expansion de le hybridomas secreting monoclonal antibodies; In vivo production de mAbs; In vitro production de mAbs; Serum-free media; Purification de Monoclonal Antibodies: Introduction; Total protein estimation methods; Proteolysis et its prevention; Purification strategy; Concentration by precipitation; Chromatography; Biomimetic ligands; Covalent affinity chromatography; Magnetic affinity beads; Protein tags; Hydrophobic interaction chromatography; Thiophilic interaction chromatography; Purification de mAbs; Purification de mAbs used pour various application; Purity testing; Protein storage; Cryopreservation de Hybridomas: Introduction; Cryopreservation; Principle de cryopreservation; Liquid nitrogen; Programmable freezers; Thawing; Selection de liquid nitrogen containers; Monitoring et replenishing liquid nitrogen; Transportation de cells; Protocol pour freezing et thawing de hybridomas; Conjugation de Monoclonal Antibodies: Introduction; Antibody modification sites; Radiolabeling de mAbs; Conjugation de mAbs to fluorescent dyes; Cross linking agents; Enzyme coupling de mAbs; Coupling de mAbs avec biotin; Coupling to magnetic particles; Detection methods pour coupled mAbs; Anti-tumour therapy using mAbs; Application de Monoclonal Antibodies: Introduction; Antigen antibody interactions–a recap; Application de mAbs en diagnosis; mAbs en immunoassays; Performance characteristics de immunoassays; Application de mAbs en antigenic characterization de viruses; Application de mAbs en detection de antibodies; Application de mAbs en detection de antigen (virus protein); Application de mAbs en detection de antigen (virus protein based quality control de vaccine; Application de mAbs en detection de antibody/antigen to viruses having multiple serotypes; Application de mAbs en differentiation de vaccinated from infected animals (DIVA); Application de mAbs en vivo depletion de T cells; Application de mAbs en aflatoxin detection; mAbs to identify receptors pour molecules; mAbs en interferon quantification et diagnosis de Mycobacterial diseases; mAbs en immunophenotyping; Application de mAbs en measuring immunoglobulin levels; mAbs et stem cells; mAbs en pregnancy diagnosis; Anti-idiotype mAbs as vaccines; mAbs en bacterial et parasitic diseases; mAbs en immunochemistry; mAbs en immunoaffinity purification; Application de mAbs as therapeutics; Ingénierie Fc region de le mAbs; Antibody drug conjugates; Radioimmunotherapy pour infectious diseases using mAbs; Single domain antibodies; Affibody; Ribosome display technology; mAbs en imaging; Intrabodies; Fc fusion proteins; Alternate hosts pour mAb production; Antibody Ingénierie: Introduction; Antibody genetics; Humanized antibodies; Prokaryotic systems; Eukaryotic systems; Recombinant expression de antibody fragments; Therapeutic antibodies; Alternatives to Monoclonal Antibodies: Introduction; Production de recombinant antibodies by phage display technology; Manipulating structural proteins de filamentous phages pour antibody libraries; Production de mono-specific sera using recombinantly expressed proteins; Steps involved en le overexpression de recombinant proteins; Production de mono-specific serum against recombinant proteins; Anti-peptide antibodies; Planteibodies; References; Suggested reading; Glossary; Appendix; Index