Agriculture Biotechnologie & Microbiologie
Manuel de Techniques en Biotechnologie
Description
This book imparts an enriching experience to le readers by providing extensive information about le techniques en biotechnology. Le fundamentals en this book are critical to le success de researchers et students alike. This book covers techniques de DNUn isolation, nucleic acid hybridization, RNUn isolation, PCR, molecular marker techniques, Immunotechniques, spectroscopy et other techniques applied en biotechnology. Le book not only provides le protocols pour various techniques but also gives theoretical information regarding it. Le principle et working de each technique clarifies le concepts et enhances understanding de le subject. Step-by-step protocols are provided along avec preparations de solutions et buffers. Numerous illustrations et diagrammatic representations de le protocol add pictorial value to le theoretical explanation. Troubleshooting guide is an invaluable tool et unique feature de this book, which will help researchers en avoiding pitfalls et obtaining targeted results. Review questions at le end de each chapter, is one more reader friendly unique feature, which will be useful pour le teachers, students et researchers alike. Le topics covered en this book are en accordance avec le postgraduate syllabi de UGC recognized universities et institutions. This book will also be helpful pour those who are preparing pour competitive exams like NET, JRF, GATE, SET, SRF etc., et pour le ones who are engaged en biotechnology research.
Table des matières
Preface; Electrophoresis: Introduction; Principle et theory de electrophoresis; Applications de electrophoresis; Agarose gel electrophoresis; SDS-PAGE electrophoresis; 2-Dimensional gel electrophoresis; Pulsed field gel electrophoresis; Isoelectric focusing; Review questions; References; Techniques de Centrifugation: Introduction; Théorie et principle de centrifugation; Types de centrifuges; Types de centrifugation techniques; Review questions; References; Spectroscopic Techniques: Introduction; UV spectroscopy; Nuclear magnetic resonance (NMR); X-ray spectroscopy; Mass spectroscopy; Review questions; References; Deoxyribonucleic Acid et Extraction de DNA: What is DNA? Its structure et properties; Enzymes that degrade DNA; Presence de DNUn en prokaryotic et eukaryotic cell; Composition de cell wall et cell membranes; Enzymes that degrade cell wall; Method pour lysing cell membranes et degrading cell walls; Protocols pour extraction et purification de DNA; Méthodes pour detection de isolated DNA; Review questions; References; Ribonucleic Acid et Extraction de RNA: What is RNA? Its structure et properties; Enzymes that degrade RNA; Types de RNUn 140; Points to remember while isolating mRNA; Creation et maintenance de RNase free working Environnement; Protocols pour extraction et purification de RNA; Méthodes pour detection de isolated RNA; Review questions; References; Radioisotopes, Radiolabelling de Probes et Autoradiography: Introduction - use de radioisotopes en biological sciences; Radioactive isotopes; Health hazards de radioactivity; Precautions et care to be taken while handling radioisotopes; Radiolabelling de probes; Technique de autoradiography; Review questions; References; Immunotechniques: Introduction: Théorie et principle de antibody based detection methods; Extraction et purification de proteins; Enzyme linked immunosorbent assay (ELISA); Radioimmunoassay; Western hybridization; Review questions; References; Techniques de Nucleic Acid Hybridization: Introduction; Use de probes en nucleic acid hybridization; Commonly used solutions et buffers en nucleic acid Hybridization; Southern hybridization; Northern hybridization; In situ hybridization; Colony hybridization; Fluorescent In situ hybridization; DNUn microarray; Review questions; References; Techniques de Chromatography: Introduction; Commonly used terms en chromatography; Théorie de chromatography; Paper chromatography; Thin layer chromatography; Affinity chromatography; Ion exchange chromatography; Size exclusion chromatography; Gas chromatography; High pressure liquid chromatography; Review questions; References; Polymerase Chain Reaction (PCR): Introduction: Théorie et principle; Pre-requisites de PCR- enzymes, dNTPs, primers; What is primer? Comparing primer, probe et gene; Comparison de PCR reaction avec en vivo DNUn replication process; Applications de PCR; Native PCR; RT- PCR; Review questions; References; Moléculaire Marker Techniques: Introduction; Importance et applications de molecular marker Techniques; Restriction fragment length polymorphism (RFLP); Random amplified polymorphic DNUn (RAPD); Amplified fragment length polymorphism (AFLP); Simple sequence repeats (SSR); Single nucleotide polymorphism (SNP); Sequence characterized amplified region (SCAR); Review questions; Reference; Subject Index